Prototyping CAPGLO: A Low-cost Magnetic Cell Capture and Imaging Platform

Abstract Magnetic cell separation has been utilized for the past 50 years for bench-top science and, more recently, in clinical applications. However, devices used to isolate cells for diagnostics and/or therapy are costly and often large, precluding easy point-of-care use. We describe a new magnetic capture device coupled with a low-cost, small-footprint imaging scope (called CAPGLO). The assembly of the device and testing of its performance are demonstrated using two sizes of fluorescent magnetic particles (MPs) and fluorescently labeled cells. Our results indicate the device is quick, easy to use, and able to image both captured MPs and cells. Also, the isolated cells proliferated normally in culture indicating that the device is able to capture cells and maintain viability. Future studies will test if the device is scalable for larger capture volumes.