Ori Refael Cohen, Dafei Xiao, Reut Orange Kedem, Onit Alalouf, Prakash Joshi, Yuya Nakatani, Anna-Karin Gustavsson, Yoav Shechtman
Point-spread-function engineering by depth-encoding phase masks enables volumetric super-resolution imaging by 3D single-molecule localization microscopy (SMLM) but usually requires cumbersome relay optics. We demonstrate simple 3D SMLM implementation by phase mask insertion directly into the infinity space of a commercial microscope, along with appropriate computational compensation for field-dependence. The entire inserted component, including the phase mask, is 3D printing-based.