On-chip lensless polarization microscope with multiplexed illumination using LED array

Polarized on-chip lensless microscopy provides a cost-effective diagnostic solution for large field-of-view (FOV) imaging. However, conventional designs often rely on a 3-dimensional and rotational motorized stage for the sensor, a configuration that necessitates an additional image registration process, increases system bulkiness, and extends image acquisition time. In this work, we present a polarized lensless microscope based on illumination diversity. The proposed system integrates a two-LED multiplexed pixel super-resolution and multi-wavelength phase retrieval framework based on an RGB-LED matrix, as well as illumination polarization-state modulation, enabling high-resolution polarized microscopic imaging without mechanical movement in the detection optics, including the specimen. Our system achieves sub-micron resolution across a wide FOV while featuring a compact, cost-effective design with rapid image acquisition and reconstruction. We expect that our proposed polarized lensless microscope design will enhance the applicability of lensless imaging systems in the biomedical field.