Analysis of mtDNA Methylation.

Whole-genome methyl sequencing facilitates the characterization of DNA methylation profiles within a reference genome. Recent identification of mitochondrial DNA (mtDNA) as an additional regulatory pathway within embryos paves the way for examining the impact of assisted reproductive technologies and other environmental factors on gamete and embryo developmental programming. Here, we describe the protocol for preparing libraries tailored to detect cytosine methylation in mtDNA extracted from minimal input, typical of experiments involving reproductive samples such as oocytes and embryos.