Duygu Nuhoğlu, Gunseli Guney Kekec, Basak Senturk, Kevser Buse Karadeniz, Eylul Ece Islek Camadan, Afranur Pendar, Yunus Zorlu, Ahmet Can Timucin, Ozge Can, Tanil Kocagoz, Cihat Taşaltın, Esra Zayim, Ilke Gurol
Copeptin is an important biomarker for the diagnosis of acute myocardial infarction in the troponin-blind period. In this study, a peptide-based nanostructured electrochemical biosensor was developed on a screen-printed electrode platform, enabling a disposable sensing strategy suitable for on-site applications. A copeptin-specific peptide, synthesized for this study, was employed as the biorecognition element to provide binding sites for the target. The UiO-66-NH2 metal-organic framework was synthesized to improve the electrochemical response and served as a functional interface due to its high surface area. The proposed biosensor platform provides a sensitive detection of copeptin with a limit of detection of 228.5 pg/mL, offering a promising low-cost and efficient peptide-based electrochemical sensing platform.