Garrett Sager, Paul E. Pfeiffer, Heng Wu, Fabian Pallasdies, Robert P. Gowers, Snusha Ravikumar, Enhua Wu, Daniel A. Colón‐Ramos, Susanne Schreiber, Damon A. Clark
connectome, we uncovered quantitative rules governing the morphology and positioning of hundreds of thousands of mitochondria across thousands of neurons. We discovered that mitochondrial morphological features are specific to cell and neurotransmitter type, which provides fingerprints to identify neurons. Mitochondria are positioned with 2- to 3-micrometer precision relative to synaptic and structural features, with systematic differences across neuron types and compartments. Mitochondrial localization correlates with regional activity and postsynaptic targets. Analysis of a mouse visual cortex connectome confirms cell type-specific morphology and identifies partially divergent positioning rules. These results establish mitochondria as circuit-embedded organelles whose distribution links subcellular architecture to brain connectivity.