Short-term L1 arrest duration does not significantly alter organismal phenotypes in synchronized Caenorhabditis elegans populations.

Synchronized L1 larvae are commonly maintained under starvation for varying durations prior to experimental use in Caenorhabditis elegans research. However, the effects of short-term L1 arrest duration on downstream phenotypes remain unclear. Here, we evaluated developmental growth, reproductive capacity, and locomotor behavior in worms derived from synchronized L1 populations maintained under starvation for 0-6 days before plating. No significant differences were observed in body length, body volume, brood size, embryonic viability, or locomotion among worms derived from different L1 arrest durations. These findings suggest that short-term L1 arrest duration did not significantly influence the organismal phenotypes evaluated in this study.