Yuzuki Nagasaka, Akihiro Isozaki, Hiroki Matsumura, Natsumi Tiffany Ishii, Norah Roels, Mina Rassuli, Kelvin C. M. Lee, Walker Peterson, Tianben Ding, Goda Keisuke
, a large microalgal cell species, based on intracellular lipid droplet formation, demonstrating 4.5-fold enrichment of target cells from 11.9% to 53.8% at 128 eps. Our work highlights that IACS based on elasto-inertial focusing enables the sorting of large objects based on high-content real-time image analysis without compromising sorting purity, yield, or event rate.