Y. Y. Li, Yuanshuo Dai, Zhigang Tang, Lijuan Chen, Junwei Zhao
Multidrug resistance genes (MDRG) overexpression represents a critical factor contributing to chemotherapeutic resistance in cancer, necessitating the development of highly sensitive detection technologies for early diagnosis. Herein, we synthesized a heterometallic decanuclear Ce III –W VI cluster incorporated antimonotungstate pentamer Na 16 H 7 [Ce 4 W 6 O 13 (H 2 O) 12 ][B-α-SbW 9 O 33 ] 5 ·40H 2 O ( 1 ) via the reaction of the [B-α-SbW 9 O 33 ] 9– precursor with Ce 3+ cations in CH 3 COOH–CH 3 COONa buffer system. Its polyanionic architecture comprises a heterometallic decanuclear [Ce 4 W 6 O 13 (H 2 O) 6 ] 22+ core encapsulated by five [B-α-SbW 9 O 33 ] 9– units. Notably, 1 represents the first reported pentamer within the rare-earth incorporated antimonotungstate family. By employing 1 as a multifunctional dopant, an electroconductive 1 @PNMPy film was fabricated through electropolymerization of N -methylpyrrole (NMPy), demonstrating enhanced conductivity and stability. The resulting 1 @PNMPy-based electrochemical DNA biosensor ( 1 -ECDB) achieves sensitive detection of MDRG, exhibiting a broad linear response range (1.0 × 10 –14 to 1.0 × 10 –7 M) and a low detection limit (4.33 fM). Moreover, the 1 -ECDB exhibits acceptable specificity, anti-interference capability, reproducibility and long-term stability. Its practical applicability has been validated in diluted human serum samples, yielding recovery rates ranging from 94.2 to 109.2%. This work pioneers the application of POM-conductive polymer composite films in the electrochemical detection of MDRG, bridging the molecular engineering of POMs with biomedical sensing technologies.