Jun Wu, Meijuan Jia, Anhua Dong, Jie Dong, Ruotong Tian, Jun Zhou, Chengrong Shen
, both MT-I and lipase are covalently immobilized onto the nanoparticle surface, creating a "gold film-MT-I/AuNPs/lipase" composite sensing interface. The experimental results indicate that the proposed sensor exhibits a sensitivity of 0.346 dB/lg(mM) and a low limit of detection (LOD) of 4.192 μM, surpassing previous reports by over 4 orders of magnitude. In addition, the lipase in the sensing membrane can catalyze the hydrolysis of triglyceride into glycerol and free fatty acids by gradually breaking the ester bonds to achieve complete hydrolysis. The "protein-nanoparticle-enzyme" synergistic sensing system established by this sensor offers valuable insights and serves as a reference for the design and development of dual-functional biosensors capable of monitoring enzymatic reactions and biosensing detection.