Yijing Xu, Ting Li, Mingquan Huang, Yunxia Luan, Jian Sun, Shan He, Huanlu Song, Mingguang Yu
The newly bred strawberry cultivar 'Jingxiang' has attracted consumer attention because of its intense aroma and pronounced peach-like notes. However, the molecular basis and ripening-associated change remain insufficiently elucidated. SPME and SAFE coupled with GC-O-QTOF were used to characterize odor-active compounds (OACs) across five ripening stages. At the full-red stage, 64 OACs were identified. Esters dominated both concentrations and flavor dilution (FD) factors, with ethyl benzoate and γ-decalactone having an FD value of 128. Moreover, furaneol had the highest FD factor (512) and was identified as a key contributor to sweetness and strawberry-like aroma. Dynamic analysis revealed substantial changes in the aroma profile during ripening, shifting from aldehyde- and alcohol-dominated green notes in the early stages to ester- and terpene-driven sweet, fruity aromas, while furanones persisted and further enhanced sweetness. This work provides potential chemical markers for flavor-oriented quality assessment, optimal harvest determination, and targeted breeding of aromatic strawberry cultivars.