Nur Kholis Firdaus, Widi Amaria, Kurnia Dewi Sasmita, Edi Wardiana, Dibyo Pranowo, Maman Herman, Muhammad Syakir, Miftakhurohmah Miftakhurohmah, Asif Aunillah, Gusmaini Gusmaini, Muhammad Firdaus Oktafiyanto, Ankardiansyah Pandu Pradana, Wahyu Wibawa
ABSTRACT Endophytic microbes play a crucial role in plant growth and health. Specifically, endophytic bacteria can be isolated from Reutealis trisperma (Blanco) Airy Shaw, a non-edible oil-producing plant prevalent across various Indonesian agroecosystems. This study aimed to select potential endophytic bacteria from R. trisperma in various Indonesian agroecosystems as plant-growth-promoting (PGP) through in vitro assays. Plant tissues were collected, and endophytic bacteria were isolated from five different R. trisperma planting areas. The bacterial colonies underwent biosafety testing; the surviving isolates were then evaluated in vitro for their ability to promote rice seedling growth, along with phytohormone analysis. The potential bacterial isolates were sequenced using 16S rRNA sequencing. The results indicated that 234 isolates passed the biosafety tests; 11 isolates were selected such as Serratia marcescens (BTB3, BNB2, KTA1, KTA5), Serratia sp. (NNB30), Klebsiella sp. (NKB2, SNA11, NTB22, GKB20), Citrobacter sp. (GKB21), and Lysinibacillus sp. (NNB25). However, isolates NTB22, NKB2, NNB30, and GKB20 have the highest potential as PGP.