A. T. Lefebvre, N. E. Steiner, S. Wang, C. L. Rodriguez, E. Bar-Kochba, Y. Shi, S.-M. Cho, D. W. Blodgett, M. Mirski
Significance: Continuous, non-invasive monitoring of cardiovascular physiology provides critical information for clinical decision-making and patient care. Emerging optical sensors enable non-contact measurement of physiological parameters such as heart rate and respiratory rate, but current methods are limited in their ability to capture spatially resolved physiological waveforms. Aim: We aimed to extend the capabilities of non-contact cardiovascular monitoring by using an imaging-based approach from which spatially resolved physiological waveforms can be extracted and cardiovascular biomarkers can be derived in vivo. Approach: We implemented a digital holographic imaging sensor to continuously measure calibrated tissue motion for in vivo assessment of cardiovascular biomarkers in six adult male Sprague-Dawley rats, with validation against electrocardiographic and invasive arterial blood pressure measurements. Results: Heart rate and pulse arrival time-derived pulse wave velocity calculated from digital holographic imaging demonstrate strong agreement with reference-derived metrics (concordance correlation coefficient [≥] 0.98). Heart rate variability shows moderate agreement with reference-derived metrics (concordance correlation coefficient [≥] 0.59).