G. J. Stevens, K. Sutinen, D. Beraldi, S. Budhanuru Ramaraju, C. M. Diesh, W. Haese-Hill, P. Xie, C. Bridge, A. Morison, A. Leung, U. Böhme, S. Cain, N. Dunn, T. Hunt, J. E. Loveland, A. Frankish, A. Papanicolau, S. Giorgetti, J. Keatley, B. Flint, L. D. Stein, R. Buels, M. Berriman, I. Holmes
We present Apollo 3, a new manual genome annotation tool that integrates with the JBrowse 2 genome browser. Its functionality is inspired by existing manual genome annotation tools such as Apollo, Artemis, and Otter, but uses an updated and more scalable architecture and technology stack. It allows the simultaneous editing of multiple genomes, including the visualization of synteny to inform those annotations. Apollo 3 can be used as a standalone annotation editor, or it can be installed on a server and used collaboratively. We describe the application's design, features, and use cases.