Aminzare Fatemeh, Mansoubi Sahar, Zare Tooranposhti Zohreh, Mohsenpour Mohaddeseh
Our findings suggest that PVT-1 lncRNA and TGF-β may serve as promising molecular biomarkers for colorectal cancer, with potential diagnostic utility. Further validation studies are warranted to establish their role in clinical practice.
INTRODUCTION: Colorectal cancer ranks among the most prevalent types of cancer across the globe, particularly in developing countries. Biomarkers like long non-coding RNAs (lncRNAs) have a key impact in early detection and personalized treatment. This study aimed to compare the expression levels of PVT-1 lncRNA and TGF-β gene in colorectal cancer tissues and non-cancerous polyp tissues.
MATERIALS & METHODS: Fifty colorectal cancer tissue samples and fifty non-cancerous polyp tissues, confirmed by a gastroenterologist, were analyzed. RNA was extracted, cDNA was synthesized, and expression levels of PVT-1 lncRNA and TGF-β were quantified using real- time polymerase chain reaction (PCR). Data analysis was performed with SPSS software, and all experiments were conducted under identical laboratory conditions to ensure reliability.
RESULTS: A total of 50 participants (22 men and 28 women) were enrolled, with a mean age of 62.56±16.41 years, while the control group consisted of 30 males and 20 females (mean age: 58.41±11.13 years). Expression analysis revealed significantly higher levels of both PVT- 1 lncRNA (fold change=X.X, P<0.05) and TGF-β (fold change=X.X, P<0.05) in colorectal cancer tissues compared to controls. These differences persisted despite demographic imbalances between groups. ROC curve analysis demonstrated the diagnostic potential of both biomarkers in distinguishing cancerous from non-cancerous tissues.
CONCLUSION: Our findings suggest that PVT-1 lncRNA and TGF-β may serve as promising molecular biomarkers for colorectal cancer, with potential diagnostic utility. Further validation studies are warranted to establish their role in clinical practice.