Sustainable Antimicrobial Textiles Functionalized with Gold and Silver Nanoparticles Biosynthesized Using Undaria pinnatifida Extracts.

Functionalizing textiles with nanoparticles is a promising strategy for developing sustainable materials with antimicrobial activity and reduced potential for resistance. This study aimed to develop antimicrobial cotton and polyester (PE) textiles functionalized with gold and silver nanoparticles (Au@UP and Ag@UP) biosynthesized using Undaria pinnatifida (UP) aqueous extracts. The nanoparticle-functionalized textiles were characterized by Ultraviolet-visible spectroscopy (UV-vis), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR), and nanoparticle attachment-detachment and stability were evaluated on both substrates. Antioxidant activity was assessed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, while antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa and cytocompatibility using the L-929 cell line were determined. UV-vis spectroscopy revealed residual release, while SEM, UV-vis, and FTIR confirmed nanoparticle binding. Cotton exhibited higher nanoparticle affinity and stability (KS = 0.5007 for Au@UP and 0.4817 for Ag@UP), attributed to its abundance of reactive hydroxyl groups. Although antioxidant activity decreased after nanoparticle binding (<5%), functionalized cotton gauzes retained antimicrobial activity. Au@UP and Ag@UP textiles were non-cytotoxic (>80% cell viability) and inhibited the growth of S. aureus (~15% and 90%, respectively) and P. aeruginosa (~90% for both). These findings support the potential of UP-mediated nanoparticle-functionalized textiles, particularly Ag@UP-containing materials, as sustainable antimicrobial surfaces.