Ailazzat A. Aitkenova, Gayane A. Atazhanova, Karakoz Z. Badekova, А. В. Самородов, Saule B. Akhmetova, Gulmira B. Shapatova, Anar K. Brazhanova, Assemay O. Imanbek, E. A. Smolyarchuk, Bibigul B. Ashirbekova
The aim of this study was to develop and characterize a soft gel dosage form based on an ethanol extract and essential oil from the rhizomes of Iris scariosa L., and to evaluate its antioxidant and anti-inflammatory activity. Plant material was extracted using ultrasonic extraction with ethanol of varying concentrations. Phytochemical composition was analyzed by HPLC and GC-MS. Carbomer-based gels were formulated and assessed for physicochemical properties, rheological behavior, stability, and release of active compounds. The highest extraction yield (21.16%) was achieved with 70% ethanol. The extract contained phenolic compounds, mainly epicatechin and gallic acid, as well as flavonoids such as naringin and phlorizin. The essential oil was rich in medium-chain fatty acids. The developed gels were transparent, homogeneous, and exhibited stable pH values within the physiological skin range, along with pseudoplastic flow behavior. Significant antioxidant activity was observed in both the extract and gel in reactive oxygen species and lipid peroxidation models. In a formalin-induced inflammation model, the gel significantly reduced paw edema. These findings demonstrate the potential of the Iris scariosa-based gel as a promising phytopharmaceutical formulation for topical application.