Haotian Wang, Runyu Wu, Lanying Li, Huimin Feng, Yixuan Zhang, Yajuan Cong, Dehai Li, Hao Liu, Meilin Zhu
To fully explore the metabolites of the mangrove endophytic fungus Penicillium janthinellum HDN13-309, which can produce Epipolythiodioxopiperazine (ETP) compounds with noteworthy anti-tumor activities, HPLC-MS analysis was applied, and five new gliovirin-like alkaloids, penicisulfuranols G-K (1-5), were target-directed acquired. Furthermore, penicisulfuranols G (1) and H (2) were distinguished by a double bond between C-5/C-6 and ortho-hydroxylation of C-7/C-8 on the 1,2-oxazadecaline moiety. Their structures, including absolute configurations, were elucidated based on NMR spectroscopy, ECD measurements, and high-resolution mass spectrometry, as well as by comparison with the literature. Penicisulfuranols I-K (3-5) showed promising cytotoxicity against four tumor cell lines (HCT116, HeLa, HL-60, and K562) with IC50 values ranging from 0.1 to 9.9 μM.