Hamed Nozari, Zornitsa Yordanova
Background: Cold chain distribution in Agri-Biotech supply chains faces serious challenges due to strict time windows, high temperature sensitivity, and conflict between different operational objectives, and conventional static approaches are unable to address these complexities. Methods: In this study, an integrated decision support framework is presented that combines multi-objective fuzzy modeling and an adaptive digital twin to simultaneously manage logistics costs, product quality degradation, and service time compliance under operational uncertainty. Key uncertain parameters are modeled using triangular fuzzy numbers, and the digital twin dynamically updates the decision parameters based on operational information. The proposed framework is evaluated using real industrial data and comprehensive computational experiments. Results: The results show that the proposed approach is able to produce stable and balanced solutions, provides near-optimal performance in benchmark cases, and is highly robust to demand fluctuations and temperature deviations. Digital twin activation significantly improves the convergence behavior and stability of the solutions. Conclusions: The proposed framework provides a reliable and practical tool for adaptive planning of cold chain distribution in Agri-Biotech industries and effectively reduces the gap between advanced optimization models and real-world operational requirements.