Micropsalliota is a genus of small, slender agaric fungi predominantly distributed in tropical and subtropical regions. To explore the species diversity of Micropsalliota in southern China, we carried out both Maximum likelihood and Bayesian phylogenetic analyses based on multi-locus sequence data (ITS, nrLSU, rpb2, and tef1-α), combined with detailed morphological observations. Based on these analyses, six new species are described: Micropsalliota alboglobulata, M. fuanensis, M. meilinensis, M. minutispora, M. pulvericlavata, and M. shenzhenensis. In addition, M. albosericea, M. gracilis, and M. purpureobrunneola are reported for the first time in China. Detailed morphological descriptions, color photographs, line illustrations, and phylogenetic trees are provided.