Integrative Taxonomy Reveals Two New Trichoderma Species and a First Mexican Record from Coffee Soils in Veracruz

Trichoderma species are globally distributed fungi with remarkable biotechnological relevance. In this study, we describe two new species, T. jilotepecense and T. sanisidroense, and report T. endophyticum as the first record for Mexico. All isolates were obtained from soils of coffee agroecosystems in Veracruz. Species identification was based on the integration of cultural and micromorphological characteristics (PDA, SNA, CMD; 25–35 °C) with multilocus phylogenetic analyses using the ITS, tef1, and rpb2 markers. The concatenated dataset provided strong support for species delimitation and clarified phylogenetic relationships within the Harzianum and Virens clades.