Tian Tu, Qi-Lei Zhang, Zhong-Ping Sun, Chao Kang, Dan Li, Xin-Hua Huo, Xiao-Jie Zhang, Yan Zhang, Mengchao Cui, Aihua Pan, Jian Wang, Xiao-Xin Yan
In the brain group with Braak/Thal 0/0 scores (control), ɑSMA labeling occurred in CA3 and dentate gyrus. In groups with 1a to IV/0 and II to VI/1 to 5 scores, ɑSMA labeling appeared in CA2 to subiculum, with increased density in these and the dentate subregions. ɑSMA-labeled MF terminals infiltrated into ghost tangles co-expressing amyloidogenic markers and coexisted among β-secreatse-1 labeled neurites and amyloid beta plaques. COX immunolabeling was increased in CA2 to subiculum in the groups with 1a to IV/0 to 1 compared to 0/0.
INTRODUCTION: Neural network and bioenergetic abnormalities occur with Alzheimer's disease (AD) pathogenesis.
METHODS: We explored mossy fiber (MF) and mitoenergetic alteration using ɑ-smooth muscle actin (ɑSMA) and cytochrome c oxidase (COX) immunolabeling in human hippocampus relative to tangle and plaque formation by referring to Braak (0-VI) and Thal (0-5) staging scores.
RESULTS: In the brain group with Braak/Thal 0/0 scores (control), ɑSMA labeling occurred in CA3 and dentate gyrus. In groups with 1a to IV/0 and II to VI/1 to 5 scores, ɑSMA labeling appeared in CA2 to subiculum, with increased density in these and the dentate subregions. ɑSMA-labeled MF terminals infiltrated into ghost tangles co-expressing amyloidogenic markers and coexisted among β-secreatse-1 labeled neurites and amyloid beta plaques. COX immunolabeling was increased in CA2 to subiculum in the groups with 1a to IV/0 to 1 compared to 0/0.
DISCUSSION: MF reorganization/dystrophy develops and integrates with tangle and plaque formation and mitoenergetic alteration in the human hippocampus.