Anna Jílková, Diana Chrpová, Adam Hruška, Andrea Maťhová, Lenka Kouřimská
Background/Objectives: University students frequently exhibit suboptimal dietary habits, and even those enrolled in nutrition-related programmes may fail to meet recommended intakes of several key nutrients. This study aimed to assess changes in dietary intake and body composition over a single academic semester among university nutrition students. Methods: A prospective pre–post study was conducted with 102 students at the Czech University of Life Sciences Prague. Dietary intake was assessed using a 3-day food record and evaluated for energy, macronutrients, and specific micronutrients. Body composition was measured by a multi-frequency bioelectrical impedance analysis. Changes between baseline and follow-up were analysed using paired statistical tests with the false discovery rate correction. Predictors of follow-up body fat percentage were examined using an analysis of covariance. Results: At baseline, mean daily energy intake was 2114 ± 632 kcal. A particularly low intake was observed for dietary fibre (15.45 ± 8.46 g/day), potassium (2013 ± 954 mg/day), iodine (63.5 ± 69.8 µg/day), and vitamin D (2.31 ± 3.01 µg/day), whereas protein intake was relatively high. During follow-up, significant increases were observed in the intake of carbohydrates (+54.2 g/day), dietary fibre (+9.3 g/day), potassium (+766 mg/day), vitamin C (+69.2 mg/day), and magnesium (+86.2 mg/day), together with lower sodium and saturated fat intake (all adjusted p < 0.001). No significant short-term changes were found in body weight, body fat percentage, or skeletal muscle mass. Follow-up body fat percentage was primarily associated with baseline adiposity. Conclusions: One semester of nutrition-related education was associated with improved dietary intake, particularly for fibre and selected micronutrients, but not with measurable short-term changes in body composition. These findings suggest that nutrition education may support healthier dietary behaviour and may contribute to preventive healthcare strategies in young adults.