Understanding the Effect of Zein-Resveratrol Interactions on the Mechanical and Functional Properties of Zein Gels.

The potential of zein gels as functional delivery platforms for resveratrol (RESV), a natural polyphenol with antiamyloidogenic action but low systemic bioavailability, is explored by a combination of spectroscopic (circular dichroism, infrared, fluorescence, UV-Vis), calorimetric (differential scanning microcalorimetry) and rheological methods. Molecular docking is also applied for mapping the interaction sites and forces between native zein and RESV. Zein-RESV interactions in solution are systematically evaluated to understand the structural changes from a soluble protein-polyphenol system to a gel network. The concentration-dependent effect of RESV on the structural and thermal stability of zein is investigated in relation to the mechanical and functional properties of zein-RESV gels. It is shown that RESV acts as a promoter of zein aggregation, with a more pronounced effect obtained at a RESV:zein 1:1 molar ratio. By increasing the α-helix content, RESV induces a more ordered zein secondary structure in solution. In gel, the three-dimensional zein network is disrupted by hydrophobic and hydrogen-bonding interactions with RESV. Fluorescence spectra of zein gels point to conformational changes upon interaction that are confirmed by infrared spectroscopy. A significant viscoelastic deformation of the gel occurs when the RESV:zein molar ratio increases to 4:1. The RESV:zein 1:1 gel network demonstrates optimal viscoelastic properties for the release of RESV, following a biphasic transition from diffusion to matrix-controlled release. The findings of this study contribute to the structural design of zein-based systems for encapsulating and delivering bioactive compounds.