Preparation-Route-Associated Differences in Color and Non-Volatile Metabolite Profiles of Eurotium cristatum-Fermented Fu Tea.

Liquid- and solid-state fermentation are both used to produce Eurotium cristatum-fermented Fu tea, but their final soluble products have rarely been compared directly. We compared a liquid-state fermented tea infusion (LFt-D7) with an aqueous extract of solid-state fermented Fu tea (SFt-D7), using an unfermented sterilized tea extract (SHt-D0) as the reference group. LFt-D7 developed a darker reddish-brown appearance and had higher theaflavin and theabrownin contents, lower thearubigin content, and larger Commission Internationale de l'Éclairage L*a*b* (CIELAB) color differences than SHt-D0 and SFt-D7. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) profiling separated the three endpoint groups; 221, 180, and 203 Level 2 annotations met the variable importance in projection (VIP > 1) and nominal p < 0.05 screening criteria in LFt-D7 versus SHt-D0, SFt-D7 versus SHt-D0, and LFt-D7 versus SFt-D7, respectively. Targeted analyses showed substantially lower free amino acid levels in SFt-D7. LFt-D7 retained a larger soluble free-amino-acid pool, had the highest total quantified flavonoid content with a stronger flavanol contribution, and showed higher relative peak-intensity signals for selected alkaloid- and phenolic acid-related metabolites. Mantel analysis showed covariation between metabolite modules and pigment indices, CIELAB parameters, total free amino acid (FAA) content, and total quantified flavonoid content. Together, these results show that the two complete preparation routes produce different soluble chemical profiles.