Effects of Dietary Quercetin Supplementation on Growth Performance, Serum Health Indices, and Whole-Blood Gene Expression in Weaned Piglets.

This study evaluated dietary quercetin supplementation to investigate the effects of increasing inclusion levels on growth performance, diarrhea, serum health-related indicators, and whole-blood gene expression in weaned piglets. In a 28-day feeding trial, 128 piglets received diets formulated to contain 0, 100, 200, or 300 mg/kg quercetin (four pens of eight piglets per treatment; pen as the experimental unit). Final body weight and average daily gain were higher in the 200 and 300 mg/kg groups than in the control, and the 300 mg/kg group had the highest gain-to-feed ratio (G:F) (p < 0.05). Diarrhea rate declined linearly (p < 0.001); the rate in the 300 mg/kg group was lower than in the control and 100 mg/kg groups but did not differ from the 200 mg/kg group. Quercetin reduced serum malondialdehyde, pro-inflammatory cytokines, urea nitrogen, and aspartate aminotransferase, while increasing total protein, albumin, total antioxidant capacity, several antioxidant enzyme activities, and interleukin-10 (p < 0.05). Serum diamine oxidase activity and D-lactate concentration also declined linearly (p < 0.001). Whole-blood expression of PTGS2 decreased; HMOX1, NFE2L2, and NQO1 increased; and SOD1 was unchanged. Overall, within the tested range, increasing dietary quercetin improved growth and feed efficiency, reduced diarrhea, and was accompanied by coordinated changes in serum health-related indicators and whole-blood transcripts.