Changxin Fan, Lele Ke, Zexiong Chen, Pin Lv
Introduction As generative artificial intelligence (GenAI) becomes increasingly integrated into higher education, greater clarity is needed regarding its impact on student learning. Methods This study conducted a three-level meta-analysis of 36 empirical studies, synthesizing 132 effect sizes from 7,229 participants. Learning outcomes were classified using the Learning Outcomes Thematic Group (LOTG) framework, and seven study-level moderators were examined. Results The results indicate a significant medium overall effect of GenAI on learning outcomes ( g = 0.499). Stronger effects were found for understanding, cognitive and creative outcomes ( g = 0.669) and higher-order learning ( g = 0.504), with moderate effects for dispositions ( g = 0.452) and attainments ( g = 0.363). Evidence was insufficient for the Using and Membership/inclusion/self-worth outcome categories. Teaching method was the only significant moderator, with collaborative learning ( g = 1.026) and blended learning ( g = 0.633) yielding the strongest effects, while other moderators showed no significant influence. Discussion These findings suggest that GenAI is most effective when embedded in interactive and collaborative pedagogies. The study introduces a GenAI-Learning Alignment Perspective and outlines implications for instructional design, assessment practices, and teacher professional development.