Genomic analysis of Botrytis cinerea causing postharvest strawberry rot and the control effect of pydiflumetofen

China exhibits the largest global cultivation area of delicious, highly nutritious small fruit, including strawberries. However, fruit storage is challenged by pathogen infection. In this study in Tai’an (117.09°E, 36.19°N), Shandong Province, China, we identified the pathogenic factors of Botrytis cinerea HM-Y1, which causes postharvest strawberry rot. We further used high-throughput sequencing to identify the main pathogens causing postharvest strawberry rot at the genus level. HM-Y1 was successfully isolated and identified as Botrytis cinerea . Spore morphology was assessed, and a multigene phylogenetic tree was constructed, including ITS , HSP60 , RPB2 , and G3PDH . To elucidate the genetic signatures of Botrytis cinerea , we analyzed the HM-Y1 genome. The total sequence length of B. cinerea HM-Y1 was found to be 43,237,401 bp, with a GC content of 41.95%. Furthermore, we found that HM-Y1 biosynthesize the terpenoid virulence factor botrydial inferred from genomic data, and that pydiflumetofen has a significant antifungal effect against this fungus, with an EC 50 value of 0.200 mg/L. The control effect of 2 mg/L pydiflumetofen on the strawberry rot disease caused by B. cinerea HM-Y1 was 66.67%. These results suggest that pydiflumetofen provides effective control for the management of postharvest fungal diseases in strawberry.