Exploring methane phenotypes and their relationship with productive traits in the Spanish Holstein Population

ratio from average gas concentrations, and 2 methane production traits (g/d) derived from the ratio. A Bayesian approach was used to estimate variance components and genetic correlations. Heritability estimates for methane phenotypes ranged from 0.07 (highest posterior density interval, HPD = 0.03: 0.11) to 0.12 (HPD = 0.06: 0.17), with moderate-to-high repeatability (0.49-0.58). The number of eructation events per minute showed higher heritability (0.30; HPD = 0.24: 0.37) and repeatability (0.75; HPD = 0.73: 0.76). The phenotypic and genetic correlations between methane concentrations traits were high, ranging from 0.78 to 0.99, except for the number of eructation events per minute, which correlated positively with peak-based traits at the phenotypic level, but lowly or even negatively at the genetic level with most methane phenotypes. The phenotypic and genetic correlation between ratio and other methane concentration traits was moderate (0.46; HPD = 0.13: 0.73) to high (0.94; HPD = 0.85: 0.99), but strong and positive (0.92; HPD = 0.84: 0.98, or 0.99; HPD = 0.98: 0.99) with methane production. Methane production traits showed high phenotypic correlation (0.56-0.74) and moderate (0.44; HPD = 0.07: 0.79) to high (0.99; HPD = 0.98: 0.99) genetic correlation with methane concentration traits. The genetic correlation estimates with productive traits were low (0.12; HPD = -0.06: 0.42) to high (0.78; HPD = 0.47: 0.98) for all methane and carbon dioxide traits, with stronger associations observed with fat yield. Sniffer devices provide robust methane phenotypes under commercial conditions, enabling accurate genetic parameter estimation. We show strong correlations among methane traits, positive links with production traits, and highlight a new methane production phenotype that avoids bias in genetic correlation estimates when productive traits are used in the conversion from parts per million to grams per day. These results support the inclusion of methane traits in breeding programs to balance productivity with environmental sustainability.