Hasibul Hasan Hredoy
The fabrication of composite synthetic vascular grafts mimicking the native extracellular matrix is highly desirable in tissue engineering. In this study, we attempted to fabricate a small-diameter vascular graft membrane composed of Polyurethane (PU), Chitosan (Ch), and Elastin (Es) using an electrospinning technique with varying rotating collector speeds. However, scanning electron microscopy (SEM) analysis revealed a complete failure of fiber formation across all rotational speeds (0, 50, 100, and 150 rpm). We hypothesize that the elevated temperature (35°C) used during the preparation of electrospinning solution components increased solvent evaporation, thereby raising the viscosity of the final composite gel to a level that caused fiber breakage during the room-temperature electrospinning process.