Manju Ne, Anuradha Natarajan, F. Sherin Rebecca, Rajamanickam Pon Nivedha, Hemalatha R, Ponnulakshmi Rajagopal, Ramya Murali, Vishnu Priya Veeraraghavan, G. Sridevi, Chella Perumal Palanisamy, Selvaraj Jayaraman
Background: Type 2 diabetes mellitus (t2dm) is a chronic metabolic disorder associated with hyperglycemia, insulin resistance, dyslipidemia, and oxidative stress, leading to multi-organ complications. Polyherbal formulations from traditional medicine are gaining attention due to their multi-target therapeutic potential and minimal side effects. Aim: To evaluate the antidiabetic, antihyperlipidemic, and antioxidant effects of seendhil polyherbal formulation (sph) in streptozotocin (stz)-induced type 2 diabetic rats. Methods: Thirty-two male wistar rats were divided into four groups: control, diabetic control, sph-treated (500 mg/kg), and metformin-treated (50 mg/kg). Diabetes was induced using a high-fat diet combined with stz injection. After 30 days of treatment, biochemical parameters including lipid profile, liver and renal markers, oxidative stress indices, and antioxidant enzyme activities were assessed. Statistical analysis was performed using one-way anova followed by duncan's multiple comparison test. Results: Diabetic rats showed significant dyslipidemia, elevated liver enzymes (alt, ast, alp), renal dysfunction markers (urea, creatinine), and increased oxidative stress markers (lpo, mda), along with reduced antioxidant enzymes (sod, cat, gpx, gst). Sph treatment significantly improved lipid profile by reducing total cholesterol, triglycerides, ldl-c, and free fatty acids while increasing hdl-c. It also restored liver and kidney function markers to near-normal levels. Furthermore, sph markedly decreased oxidative stress and enhanced antioxidant defense systems. Conclusion: Seendhil polyherbal formulation exhibits significant antidiabetic, hepatoprotective, nephroprotective, and antioxidant effects in experimental t2dm, supporting its potential as a safe and effective alternative therapy.