Rasmiati Rasmiati, Christine Anastasia Rovani, Juni Jekti Nugroho
This project outlines a standardized workflow for formulating and validating a novel bio-hybrid endodontic irrigant combining ferulic acid (Schizostachyum brachycladum), saponins (Sapindus rarak), and monomeric glucosamine from tiger prawn shell chitosan. It integrates precursor chemical purity verification, post-obturation dentin collagen tracking via FTIR, and a single-sample sequential correlative microscopy framework (wet CLSM followed by dry carbon-coated SEM-EDS) for evaluating smear layer removal, tubule penetration, and Ca/P biomineralization at the dentin-sealer interface.