Naoluotong granules inhibit necroptosis of neural cells and improve cerebral ischemia-reperfusion injury by regulating the RIP1/NIK/IKKα/NF-κB pathway.

BACKGROUND: Cerebral ischemia-reperfusion can cause structural changes in brain tissue and disrupt physiological functions. Naoluotong granules (NLT) exhibit significant neuroprotective effects on brain nerve cells and are widely utilized in treating cerebral ischemia-reperfusion injury (CIRI). However, the underlying molecular mechanisms remain elusive. METHODS: , an oxygen-glucose deprivation/reoxygenation (OGD/R) PC12 cell model was established and treated with the drug-containing serum of NLT and AST-IV. RESULTS: studies also showed that NLT and AST-IV reduced OGD/R-induced necroptosis. CONCLUSIONS: This study confirms that the significant neuroprotective effects of NLT and AST-IV in alleviating CIRI are related to inhibiting necrosis by suppressing the RIP1/NIK/IKKα/NF-κB pathway.