Victoria Knight, Nils Bode, Dara P. Dowlatshahi, Jung-Gun Kim, Mary Beth Mudgett, Soichi Wakatsuki, Adam J. Bowman, Mark A. Kasevich
We demonstrate volumetric fluorescence lifetime microscopy (FLIM) using the electro-optic FLIM technique. Images acquired in a selective plane illumination microscope are gated using a Pockels cell driven at 80 MHz, enabling light sheet electro-optic FLIM (LS-EO-FLIM) acquisition with up to 800 μ m field of view. Volume acquisitions are demonstrated on fluorescent bead mixtures and in live Arabidopsis thaliana root samples using both genetically encoded fluorescent proteins and endogenous autofluorescence.