Robert Heizelman, Yash Dixit, Derek Kluczynski, Jenna Bearden, Caitlin Stidam, Zach Madaras, Meghana Perumalla, Lydeyiah Roesner, Waleed Hamdan, Samantha Schon, Nagalakshmi Nadiminty, Tariq Shah, Puneet Sindhwani, Tomer Avidor-Reiss
Infertility impacts one in seven couples globally, with one-third of cases classified as unexplained. One source of unexplained infertility is the spermatozoon centrosome, a subcellular structure composed of two remodeled centrioles (the proximal and distal centrioles) and specialized pericentriolar material, located in the spermatozoon neck. The centrosome functions as the head-neck linker in the spermatozoa. Here, we investigated the localization of the somatic cell centriole lumen protein POC1A using immunofluorescence and confocal microscopy. We found that POC1A localizes to the neck, colocalizing with POC1B, and is present in both the proximal and distal centrioles in both human and bovine spermatozoa.