Evaluating nongenetic maternal transmission and post-weaning persistence of gut microbiota in cross-fostering rabbits

Abstract Gut microbiota is widely recognized as an important source contributing to phenotypic variation of diverse host traits in livestock. In this regarding, it is promising to improve offspring production traits through direct selection on parental individuals with the desirable gut microbiota, and this is termed microbiome breeding. However, the extent of nongenetic maternal transmission of gut microbiota and its post-weaning persistence remains poorly understood. To fill this gap, we cross-fostered 120 newborn rabbits that are offspring of 20 does in this study, and compared gut microbiota of all kits at the weaning (35 days of age) and two weeks post-weaning, respectively. Based on variance component analysis, we found that the large proportions of phenotypic variation of four alpha diversity metrics for kits at weaning can be explained by the nursing does, ranging from 0.218 ± 0.106 for Evenness index to 0.464 ± 0.125 for the number of observed features. The significant effect of nursing does was supported further by comparing three beta diversity metrics among different relationships, using Kruskal-Wallis test and Dunn’s post-hoc comparisons. In contrast, the biological does showed no significant effect on gut microbiota composition of pre-weaning kits. In post-weaning kits, the influence of nursing does on gut microbiota was not obviously decreased, despite there was increasing contributions of additive genetic effects. In conclusion, this study provides direct evidence in rabbits that offspring gut microbiota is predominantly shaped by the nongenetic maternal transmission, with these maternal influences persisting post-weaning. These results indicate the possibility that the direct selection on parental gut microbiota will alter offspring gut microbiota.