Disruption of the reciprocal inhibitory relationship between cell proliferation and melanogenesis in pet murine melanoma cells by ultraviolet radiation.

This study investigates the relationship between intracellular eumelanin content and proliferation of melanoma cells derived from pet murine under ultraviolet (UV) radiation. B16F10 cells, a murine melanoma cell line established from pet murine models, were exposed to UV doses ranging from 0 to 2,880 J/m2. Cell proliferation was assessed using the CCK-8 assay, while intracellular eumelanin content was quantified via microplate reader after cell lysis, normalized using BCA protein quantification. Linear regression was performed using partial regression analysis revealed a positive correlation between eumelanin content and cell proliferation at low-to-moderate UV doses. However, this linear relationship was disrupted at the highest dose. Protein-protein interaction (PPI) analysis further demonstrated that PIAS3, a regulatory protein, modulates both STAT3 and MITF, key factors in melanoma progression. These findings suggest that UV exposure enhances eumelanin production and proliferation in pet murine melanoma cells at sub-lethal doses, but excessive UV exposure may disrupt this balance, potentially through downregulation of PIAS3. The results highlight the dual role of UV radiation in promoting eumelanin synthesis as a protective mechanism while also driving tumor growth, offering insights into the pathogenesis of melanoma in pet murine and underscoring the importance of UV protection in their clinical management.