The Nonhuman Primate Developmental Genotype-Tissue Expression Biospecimen and Histopathology Resource.

INTRODUCTION: The NIH-funded initiative Developmental Genotype-Tissue Expression (dGTEx) is an ongoing large resource of nonhuman primate (NHP) samples in which multiple organs are being collected, subdissected and preserved to build a unique tissue biorepository. Primates share close evolutionary relationships with humans, allowing for strong translation of research findings to human health. OBJECTIVE: Generate a resource consisting of the two most frequently used NHP species in research the rhesus macaque (Macaca mulatta), which is widely available and has a well-studied genome, and the common marmoset (Callithrix jacchus), which is favored for studies of brain development and neurodegenerative disease. METHODS: The dGTEx project is building (1) a genetic repository that will provide genome and tissue-based RNA sequencing for these Old and New World primate species to identify how genetic variations affect normal gene expression and to understand mechanisms of disease, and (2) an expansive histopathology resource of preserved individual organs. Detailed animal history, demographics, and sample metadata are documented in a secure database. Samples are being obtained from first (rhesus only), second, and third trimester fetal cohorts (with accompanying gestational tissues) as well as neonatal, infant, juvenile, and adult animal timepoints balanced for male and female to facilitate sex-specific data analysis. RESULTS: We detail the sampling sites, preservation methods, and accompanying histology review that allows for confirmation of tissue type and quality, in addition to noting any pathological findings. CONCLUSION: This comprehensive NHP resource enables developmental investigations across the lifespan, from prenatal and postnatal stages through to adulthood.