Jing Wang, Xiaoyu Yang, Xuemei Gao, Dong Li, Zhuhua Yao
Therefore, it is speculated that GPR55 alleviates DCM by inhibiting the TNF-α-mediated RhoA/ROCK2/IκBα/p65 signaling pathway, which highlights the potential role of GPR55 in the treatment of DCM, and provides basic data to support future studies.
BACKGROUND: Challenges remain to the pathogenesis and targeted intervention of diabetic cardiomyopathy (DCM). G protein-coupled receptor 55 (GPR55) plays an important role in the onset and progression of DCM, however, the regulatory mechanism remains unknown.
METHODS: In this study, we successfully induced DCM in rats by intraperitoneal injection of streptozotocin and explored the efficacy of GPR55 in ameliorating DCM through the intervention of GPR55 activator O-1602.
RESULTS: The results showed that the intervention of O-1602 alleviated pathological lesions in cardiac tissues of DCM rats, reduced blood glucose levels, inhibited oxidative stress and suppressed inflammatory pathways. In addition, in vitro H9C2 cell assay confirmed the ameliorative effect of O-1602. The mechanism of DCM alleviation by GPR55 revealed the contribution of the inhibited RhoA/ROCK2/IκBα/p65 signaling pathway.
CONCLUSION: Therefore, it is speculated that GPR55 alleviates DCM by inhibiting the TNF-α-mediated RhoA/ROCK2/IκBα/p65 signaling pathway, which highlights the potential role of GPR55 in the treatment of DCM, and provides basic data to support future studies.