C. Cohen, Jolet Y. Mimpen, Alina Kurjan, Claudia Paul, S. Sharma, Lorenzo Ramos-Mucci, Chinemerem T. Ikwuanusi, Ali Cenk Aksu, Tracy Boakye Serebour, Marina Nikolic, Kévin Rue-Albrecht, C. L. M. H. Gibbons, Duncan Whitwell, Thomas Cosker, Stephen Gwilym, Ather Siddiqi, Raja Bhaskara Rajasekaran, Harriet Branford-White, A P Cribbs, P A Hulley, David Sims, Mathew Baldwin, Sarah Snelling
We present the first spatially resolved single-cell atlas of the human Achilles tendon.
We present the first spatially resolved single-cell atlas of the human Achilles tendon. By sampling across the microanatomy of the tendon from enthesis to muscle, we demonstrate changes in fibroblast composition across this transitional tissue. Distinct fibroblast subsets were discovered with specific transcriptomic signatures, and several were found in distinct spatial locations corresponding to putative functional roles in tendon and adjoining muscle. These findings demonstrate how cellular diversity across a transitional tissue may underlie microanatomical-specific roles.