Yifeng Wang, Jiezheng Ying, Jia Li, Xinyong Liu, Qianya Zhou, Shuang Jia, Guanghao Li, Wanning Liu, Zhenghao Tao, Hongzhou Li, Zhiyong Li, Jie Huang, Xiaohong Tong, Xixi Liu, Yu Cheng, Yuxuan Hou, Guangheng Zhang, Yangjun Zhang, Kun Zhou, Xiaoshan Zeng, Jian Zhang
Seed longevity (SL) is vital for ensuring food security worldwide. However, the genetic basis of SL has been scarcely documented. Here, we report the cloning of a major SL locus, qSL6, encoding a fatty acyl-ACP thioesterase type B. SL6 is functionally conserved in regulating palmitic acid synthesis in seeds, conferring higher oxidation durability and SL in various species. Through the VP1-SL6 module, a seed desiccation-derived ABA signal is transmitted via VP1, which directly activates SL6 transcription to alter the fatty acid composition and elevate SL in seeds. The ancestral elite allele SL6HHZ harbors a virus-derived CT-rich motif cis-element in the 5'UTR, which serves as a universal, bidirectional mRNA stabilizer, contributing to the divergence between indica and japonica in terms of SL. Moreover, manipulating SL6 expression via marker-assisted selection or transgenic approaches notably improved SL in rice cultivars and F1 hybrids without affecting major agronomic traits. Our findings provided a promising genetic locus for improving SL in rice.