Ishani Banik, Sugandha Basu, Meng Wang, Austin J Escobar, Veronica Steri, Juan A Camara Serrano, Paul Phojanakong, Noel Cruz Pacheco, Katy K Tsai, Edwin A Alvarez, Adil Daud, Ivan H El-Sayed, Anastasia Samarkina, Joy Xu, Jamie D Aquino, Min Xiao, Meenhard Herlyn, Iwei Yeh
Mucosal and acral melanomas are rare subtypes of melanoma with unique clinical and biological features. A dearth of well-established and characterized melanoma cell lines that reflect rare subtypes impedes research on these lethal diseases. We present a protocol developed by exploring various combinations of established techniques that we used to generate mucosal and acral cell lines from patient tissues and patient derived xenografts.