Mengdi Shan, Gaozhen Jiao, Xinming Liu, Xicheng Dai, Qingxi Chen, Yan Lei, Zhifeng Wen
ABSTRACT Anthracnose, caused by Colletotrichum species, severely threatens grape production during fruit ripening. This study identified the causal agent from symptomatic grapes in Fujian as C. fruticola ( C. gloeosporioides complex) through multilocus phylogeny (ITS, TUB2 , ACT ) and morphological analysis. Resistance screening of 52 Vitis genotypes via leaf inoculation revealed that V. davidii ‘Ziqiu’ and V. pseudoreticulata exhibited complete resistance, followed by nine moderately resistant cultivars including V. amurensis ‘Zuoshan‐1’ and V . vinifera 'Cabernet Sauvignon'. Among six fungicides tested, carbendazim (EC 50 = 0.23 mg/L) and prochloraz (EC 50 = 0.39 mg/L) demonstrated superior efficacy, while thiram (EC 50 = 53.38 mg/L) and mancozeb (EC 50 = 63.32 mg/L) showed limited control. These findings provide the first report of C. fruticola ‐induced grape anthracnose in southeastern China, along with validated germplasm resources and integrated disease management strategies. However, the study is constrained by its reliance on a single pathogen isolate and in vitro assay conditions.