Emmanuel L. Bernardo, Cristina Rodrigues Gabriel Sales, Tianshu Sun, Johannes Kromdijk
Summary Stomata have the crucial role of balancing the uptake of CO 2 for photosynthesis and water loss via transpiration by adjusting their aperture. In C 3 plants, coordination between photosynthesis and stomatal conductance can be disrupted by mutations in Calvin–Benson–Bassham (CBB) cycle expression, but it is not clear whether the disruption of the C 4 carbon concentrating mechanism would have similar effects. In this study, it was hypothesized that perturbing the C 4 cycle, resulting in reduced carbon flux into the bundle sheath cells and overall net CO 2 assimilation rates, may alter stomatal regulation. To test this hypothesis, we performed gas exchange measurements on transgenic Flaveria bidentis plants carrying an antisense construct leading to reduced nicotinamide adenine dinucleotide phosphate (NADP)‐malic enzyme (NADP‐ME) activity. Stomatal conductance was unaltered in the antisense plants at ambient CO 2 levels. However, C i was significantly increased. When measurements were instead performed at common C i , stomatal conductance was significantly higher in the antisense lines. These results demonstrate that disruption of the C 4 cycle via reduced NADP‐ME activity in F. bidentis leads to altered stomatal sensitivity to C i .