Nan Chai, Wuge Liu, Kangli Sun, Qian Qian, Yao‐Guang Liu, Xianrong Xie, Qinlong Zhu, Jiantao Tan
Exo-Cas12i2 v1, a fusion of the 5' exonucleases T5E and PapE, facilitates editing of TA-rich regions and mediates deletions of large genomic fragments. Exo-Cas12i2 v1-driven MITE manipulation enables precise regulation of genes involved in gibberellin-mediated cell elongation and root ethylene responses to generate favorable agronomic traits.