Fengxue Jing, Leping Geng, Yanyan Zhang, Hongtao Xie, Jian‐Kang Zhu, Jianhua Zhu
A stable and efficient transformation system is crucial for functional genomics and trait improvement in soybean. This study developed a tissue culture based genetic transformation system incorporating dual selection (Spectinomycin and RUBY). This system significantly enhances transformation efficiency, shortens the transformation cycle, and demonstrates broad genotype independence, providing a powerful tool for soybean research and breeding.