Juncheng Yan, Bingpeng Liu, Hui Lu, Shuai Zhan, Dongbin Chen, Jun Xu
A CRISPR/Cas9 toolkit was established for the rice leaffolder (Cnaphalocrocis medinalis) using entirely endogenous regulatory elements. An all-in-one plasmid was constructed containing an IE1-EGFP marker, a CmU6 promoter driven sgRNA targeting Cmebony, and a Cmactin5C promoter driven Cas9. This platform provides a system for functional genomics in this pest.