Construction of Primers for DNA-Based Storage Systems.

To enable random access in artificial DNA-based storage systems, it is essential to develop a balanced and stable address sequence with minimal cross-correlation, ensuring address independence. The goal of our paper is to design primers suitable for use as addresses within these systems. We extend the construction of secondary structure-avoiding DNA codes from [1] and modify these codes to create primers that meet the necessary specifications. To achieve the desired functionality, the paper presented three methods of primer construction. The first method generates the set of 138 primer sequences of length 64 which are mutually uncorrelated, while the other constructions generate a much higher number of primer sequences with weak uncorrelatedness. Finally, the paper concludes with the analysis and the examples for each construction. We also stimulated the primers under insertion, deletion, and substitutuion channels and also provided a further direction for the scheme.