GENETHOFF: a flexible workflow for genome wide profiling of CRISPR/Cas off targets

We developed GENETHOFF, a flexible single-command versatile Snakemake workflow designed for the comprehensive analysis of CRISPR/Cas9 related OFF-targets genomic positions from GUIDE-Seq derived protocols. It efficiently processes multiplexed libraries from different organisms, PCR orientations, and Cas nucleases with varying PAM specificities in a single run, all based on a simple user-specified datasheet containing sample metadata.