We report a case of enterogastric bile reflux (EGBR) identified in a 60-year-old man during a 99mTc-Sestamibi myocardial perfusion SPECT scan. Due to its hepatobiliary clearance being analogous to that of 99mTc-EHIDA, 99mTc-Sestamibi could help to detect EGBR, which highlights its potential as an additional diagnostic tool.